Method to eliminate linear DNA from mixture containing nicked circular, supercoiled, and linear plasmid DNA
نویسندگان
چکیده
منابع مشابه
Escherichia coli helicase II (uvrD) protein can completely unwind fully duplex linear and nicked circular DNA.
We have examined the duplex DNA unwinding (helicase) properties of the Escherichia coli helicase II protein (uvrD gene product) over a wide range of protein concentrations and solution conditions using a variety of duplex DNA substrates including fully duplex blunt ended and nicked circular molecules. We find that helicase II protein is able to initiate on and completely unwind fully duplex DNA...
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Recombinant plasmids pK1A108, pK3A108, pK4A108 and pK5/6T217 containing 80 +/- 1 base pair inserts with different curvature-inducing sequences were studied using the DNA structure probe osmium tetroxide in the presence of pyridine (Os, py). The insertion sequences of the plasmids pK1A108, pK3A108, and pK4A108 are strongly related while the degree of curvature increases from pK1A108 (no curvatur...
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Proton NMR experiments on an intact plasmid supercoiled DNA molecule are reported. Spectra of the hydrogen-bonded exchangeable imino protons and nonexchangeable aromatic protons of pIns36 (7,200 base pairs; Mr 5 x 10(6)) were examined at 240 and 360 MHz. In contrast to linear DNA of comparable size in which the signals from these protons are broadened almost beyond detection, the supercoiled DN...
متن کاملSpatial confinement induces hairpins in nicked circular DNA
In living cells, DNA is highly confined in space with the help of condensing agents, DNA binding proteins and high levels of supercoiling. Due to challenges associated with experimentally studying DNA under confinement, little is known about the impact of spatial confinement on the local structure of the DNA. Here, we have used well characterized slits of different sizes to collect high resolut...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2008
ISSN: 0003-2697
DOI: 10.1016/j.ab.2008.06.037